全文获取类型
收费全文 | 7538篇 |
免费 | 1044篇 |
国内免费 | 540篇 |
出版年
2024年 | 6篇 |
2023年 | 157篇 |
2022年 | 107篇 |
2021年 | 226篇 |
2020年 | 316篇 |
2019年 | 368篇 |
2018年 | 352篇 |
2017年 | 337篇 |
2016年 | 318篇 |
2015年 | 299篇 |
2014年 | 367篇 |
2013年 | 686篇 |
2012年 | 258篇 |
2011年 | 335篇 |
2010年 | 264篇 |
2009年 | 378篇 |
2008年 | 383篇 |
2007年 | 372篇 |
2006年 | 365篇 |
2005年 | 346篇 |
2004年 | 310篇 |
2003年 | 306篇 |
2002年 | 285篇 |
2001年 | 214篇 |
2000年 | 143篇 |
1999年 | 179篇 |
1998年 | 135篇 |
1997年 | 127篇 |
1996年 | 120篇 |
1995年 | 107篇 |
1994年 | 106篇 |
1993年 | 118篇 |
1992年 | 94篇 |
1991年 | 76篇 |
1990年 | 59篇 |
1989年 | 41篇 |
1988年 | 44篇 |
1987年 | 45篇 |
1986年 | 31篇 |
1985年 | 48篇 |
1984年 | 55篇 |
1983年 | 40篇 |
1982年 | 65篇 |
1981年 | 37篇 |
1980年 | 30篇 |
1979年 | 27篇 |
1978年 | 20篇 |
1977年 | 10篇 |
1976年 | 2篇 |
1973年 | 5篇 |
排序方式: 共有9122条查询结果,搜索用时 15 毫秒
61.
Four series of para or meta - substituted thiazolylbenzenesulfonamides bearing Cl substituents were designed, synthesized, and evaluated as inhibitors of all 12 catalytically active recombinant human carbonic anhydrase (CA) isoforms. Observed affinities were determined by the fluorescent thermal shift assay and the intrinsic affinities were calculated based on the fractions of binding-ready deprotonated sulfonamide and CA bearing protonated hydroxide bound to the catalytic Zn(II) in the active site. Several compounds exhibited selectivity towards CA IX, an anticancer target. Intrinsic affinities reached 30 pM, while the observed affinities - 70 nM. The structure-intrinsic affinity relationship map of the compounds showed the energetic contributions of the thiazole ring and its substituents. 相似文献
62.
《Animal : an international journal of animal bioscience》2015,9(1):76-85
Selection programs have enabled broiler chickens to gain muscle mass without similar enlargement of the cardiovascular and respiratory systems that are essential for thermoregulatory efficiency. Meat-type chickens cope with high ambient temperature by reducing feed intake and growth during chronic and moderate heat exposure. In case of acute heat exposure, a dramatic increase in morbidity and mortality can occur. In order to alleviate heat stress in the long term, research has recently focused on early thermal manipulation. Aimed at stimulation of long-term thermotolerance, the thermal manipulation of embryos is a method based on fine tuning of incubation conditions, taking into account the level and duration of increases in temperature and relative humidity during a critical period of embryogenesis. The consequences of thermal manipulation on the performance and meat quality of broiler chickens have been explored to ensure the potential application of this strategy. The physiological basis of the method is the induction of epigenetic and metabolic mechanisms that control body temperature in the long term. Early thermal manipulation can enhance poultry resistance to environmental changes without much effect on growth performance. This review presents the main strategies of early heat exposure and the physiological concepts on which these methods were based. The cellular mechanisms potentially underlying the adaptive response are discussed as well as the potential interest of thermal manipulation of embryos for poultry production. 相似文献
63.
Rajan Kumar Choudhary Vikrant Quadir M. Siddiqui Pankaj S. Thapa Sweta Raikundalia Nikhil Gadewal 《Journal of biomolecular structure & dynamics》2016,34(7):1533-1544
BARD1–BRCA1 complex plays an important role in DNA damage repair, apoptosis, chromatin remodeling, and other important processes required for cell survival. BRCA1 and BARD1 heterodimer possess E3 ligase activity and is involved in genome maintenance, by functioning in surveillance for DNA damage, thereby regulating multiple pathways including tumor suppression. BRCT domains are evolutionary conserved domains present in different proteins such as BRCA1, BARD1, XRCC, and MDC1 regulating damage response and cell-cycle control through protein–protein interactions. Nonetheless, the role of BARD1BRCT in the recruitment of DNA repair mechanism and structural integrity with BRCA1 complex is still implicit. To explicate the role of BARD1BRCT in the DNA repair mechanism, in silico, in vitro, and biophysical approach were applied to characterize BARD1 BRCT wild-type and Arg658Cys and Ile738Val mutants. However, no drastic secondary and tertiary structural changes in the mutant proteins were observed. Thermal and chemical denaturation studies revealed that mutants Arg658Cys and Ile738Val have a decrease in Tm and ?G than the wild type. In silico studies of BARD1 BRCT (568-777) and mutant protein indicate loss in structural compactness on the Ile738Val mutant. Comparative studies of wild-type and mutants will thus be helpful in understanding the basic role of BARD1BRCT in DNA damage repair. 相似文献
64.
Stanton E. Tuller 《International journal of biometeorology》1997,41(1):26-33
Afternoon observations in summer comparing shoreline with inland atmospheric conditions were made during onshore winds at
Victoria, British Columbia, Canada. The onshore wind came from a cool water surface. Mean monthly water temperatures near
to shore were between 11 and 11.5° C. The onshore wind brought lower air, ground surface radiant and sky radiant temperatures;
lower humidity and greater wind speed. All of these combine to produce a cooler human environment at the shoreline than inland.
The relative importance of climatic elements in producing the cooler environment was assessed using sensitivity analyses with
eight different human thermal exchange models/indices. Air temperature and wind speed had the greatest effect, followed by
ground surface radiant temperature, sky radiant temperature and humidity. Wind speed is the most practical element to consider
when trying to maximize human comfort along the shoreline.
Received: 9 July 1996 / Revised: 31 March 1997 / Accepted: 14 April 1997 相似文献
65.
This paper addresses the stability problem on the memristive neural networks with time-varying impulses. Based on the memristor theory and neural network theory, the model of the memristor-based neural network is established. Different from the most publications on memristive networks with fixed-time impulse effects, we consider the case of time-varying impulses. Both the destabilizing and stabilizing impulses exist in the model simultaneously. Through controlling the time intervals of the stabilizing and destabilizing impulses, we ensure the effect of the impulses is stabilizing. Several sufficient conditions for the globally exponentially stability of memristive neural networks with time-varying impulses are proposed. The simulation results demonstrate the effectiveness of the theoretical results. 相似文献
66.
《Current biology : CB》2021,31(23):5385-5392.e4
67.
68.
《Bioscience, biotechnology, and biochemistry》2013,77(4):716-721
Ricin A-chain, a protein that inactivates ribosomes by a specific RNA N-glycosidase activity, has been shown to be inactivated by chemical modification of a few arginine residues. When two or fewer arginine residues in the A-chain were modified with [14C]phenylglyoxal, arginines at positions of 193, 196, 213, and 234/235 were found to be modified, from amino acid compositions and radioactivities of the modified peptides that were obtained by cyanogen bromide cleavage followed by tryptic and chymotryptic digestion. All these arginines have side chains outside the active site cleft; the side chain of Arg213 is adjacent to the edge of the cleft, while other modified arginines are located on the opposite side of the cleft. Kinetic analysis showed that the modification of two arginine residues caused a 8-fold loss in kcat with a 3-fold increase in Km, suggesting that this modification mainly decrease the rate of depurination with an additional effect on the affinity for ribosomes. Neither the environment of tryptophan 211 at the bottom of the cleft nor an interaction of adenine with the cleft was changed by this modification, as judged by fluorescence spectroscopy, suggesting that a conformational change of the catalytic site does not occur upon the modification. These results, taken together with other works, suggest that some of the above arginine residues outside the active site cleft may additively contribute to the catalysis of depurination and/or the initial formation of the A-chain/ribosome complex. 相似文献
69.
The microbial production of dextranase using cheap carbon sources is beneficial to solve the economic loss caused by the accumulation of dextran in syrup. A food-grade microbial cell factory was constructed by introducing the dextranase encoding gene DEX from Chaetomium gracile to the chromosome of Bacillus subtilis, and the antibiotic resistance marker gene was subsequently deleted via the Cre/loxP strategy. The dual-promoter system with a sequentially arranged constitutive P43 promoter resulted in an 85 % increase in DEX expression. Under the optimal fermentation conditions of 10 g/L maltose, 15 g/L casein, 1 g/L Na2HPO4, 1 g/L FeSO4 and 8 g/L NaCl, DEX activity was increased from 2.625 to 64.34 U/mL. Recombinant DEX was purified 5.98-fold with a recovery ratio of 26.67 % and specific activity of 3935.02 U/mg. Enzyme activity was optimal at 55 °C and pH 5.0 and remained 80.34 % and 71.36 % of the initial activity at 55 °C and pH 4.0 after 60 min, respectively. The enzyme possessed high activity in the presence of Co2+, while Ag+ showed the strongest inhibition ability. The optimal substrate was 20 g/L dextran T-2000. The findings could facilitate the low-cost, large-scale production of food-grade DEX for use in the sugar industry. 相似文献
70.
《Journal of biological dynamics》2013,7(2):440-456
A four-dimensional food-web system consisting of a bottom prey, two middle predators and a generalist predator has been developed with modified functional response. The system is well posed and dissipative. Some results on uniform persistence have been developed. The dynamics of the system is found to be chaotic for certain choice of parameters. The coexistence of all four species is possible in the form of periodic orbits/strange attractors for suitably chosen set of parameters. 相似文献